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白介素29（干擾素λ，人類，rDNA衍生）WHO參考試劑

??【編號】：NIBSC10/176

??【名稱】：白介素29（干擾素λ，人類，rDNA衍生）WHO參考試劑

??【規(guī)格】：個

??【價格】：2600元

　　白介素29（干擾素λ，人類，rDNA衍生）WHO參考試劑

　　編號：NIBSC10/176

　　英文：Interleukin-29 (Interferon Lambda, Human, rDNA derived) WHO Reference Reagent

　　標準類型：非WHO參考資料

　　規(guī)格：1個

　　描述：1. INTENDED USE

This material is intended as a biological reference standard in bioassays

for IL-29 (Interferon Lamda 1).

2. CAUTION

This preparation is not for administration to humans or animals in

the human food chain.

The preparation contains human and bovine source material. The

preparation contains an excipient of human origin which has been

tested and found negative for HBsAg and HIV antibody. The

preparation has subsequently been tested and found negative for antiHCV

and HCV RNA by PCR. The bovine casein used as an excipient

was sourced from a country where bovine spongiform encephalopathy

(BSE) has not been found. As with all materials of biological origin,

this preparation should be regarded as potentially hazardous to health.

It should be used and discarded according to your own laboratory's

safety procedures. Such safety procedures should include the wearing

of protective gloves and avoiding the generation of aerosols. Care

should be exercised in opening ampoules or vials, to avoid cuts.

3. UNITAGE

The assigned potency of the preparation is 5,000 units of biological

activity per ampoule.

4. CONTENTS

Country of origin of biological material: United Kingdom.

Each ampoule contains the residue after freeze-drying of 1ml of

phosphate buffered saline that contained:

Approximately 500 nanograms of recombinant human IL-29

1.0% human serum albumin

0.3% bovine casein

The IL-29 protein was expessed in E.coli.

The ampoule does not contain bacteriostat.

5. STORAGE

For economy of use, it is recommended that the solution be sub-divided

into several small aliquots and stored at -40oC or below. Avoid repeated

thawing/freezing. Unopened ampoules should be stored at -20oC.

Please note: because of the inherent stability of lyophilized material,

NIBSC may ship these materials at ambient temperature.

6. DIRECTIONS FOR OPENING

DIN ampoules have an 'easy-open' coloured stress point, where the

narrow ampoule stem joins the wider ampoule body. Various types of

ampoule breaker are available commercially. To open the ampoule,

tap the ampoule gently to collect material at the bottom (labelled) end

and follow manufactures instructions provided with the ampoule

breaker.

7. USE OF MATERIAL

No attempt should be made to weigh out any portion of the freeze-dried

material prior to reconstitutionDissolve the total contents of the ampoule with 0.5ml of sterile distilled

water. Rinse the ampoule with 0.4ml of sterile distilled water and make up

the total volume to 1ml with sterile distilled water. This solution should

now contain IL-29 at a concentration of 5,000 U/ml. Use carrier protein

where extensive dilution is required. It is recommended that the IL-29

solution be used immediately or stored as indicated in section5.

8. STABILITY

Reference materials are held at NIBSC within assured, temperaturecontrolled

storage facilities and they should be stored on receipt as

indicated on the label. It is the policy of WHO not to assign an expiry date

to their international reference materials. Accelerated degradation studies

have indicated that this material is suitably stable, when stored at -20?C

or below, for the assigned values to remain valid until the material is

withdrawn or replaced. These studies have also shown that the material

is suitably stable for shipment at ambient temperature without any effect

on the assigned values. Users who have data supporting any

deterioration in the characteristics of any reference preparation are

encouraged to contact NIBSC.

9. REFERENCES

This reference reagent was produced under WHO guidelines cited in the

WHO Technical Reports Series, no. 925, 2005.

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